Multiskan SkyHigh Microplate Spectrophotometer

Overview

Overview

Thermo Scientific Multiskan SkyHigh Microplate Spectrophotometer is an easy to use instrument for photometric and turbidimetric applications, especially for DNA, RNA and protein analyses, including endpoint, kinetic, and spectral assays. Results are shown on Multiskan SkyHigh models with a large high-resolution touch screen. SkanIt Software is a powerful tool to address the challenging applications.

Multiskan SkyHigh Microplate Spectrophotometer is a UV/Vis microplate reader designed to be convenient and easy to use for any photometric absorption or turbidimetric research applications and especially DNA, RNA and protein analysis. This microplate reader is ideal for multi-user environments where a variety of endpoint, kinetic and spectral assays are performed. Multiskan SkyHigh is available in different configurations. Touchscreen models offer the flexibility to use the system as stand-alone, or together with Thermo Scientific™ SkanIt Software for PC control. A model with a cuvette reading option is also available. For convenient data handling, Multiskan SkyHigh has an option to access to Thermo Fisher Connect and Microsoft OneDrive cloud-based tools.

• Monochromator-based optical system for free selection of wavelengths from 200 nm to 1000 nm
• Compatible with 6-48, 96- and 384-well microplates or cuvettes with maximum plate height of 19.5 mm including possible lid
• Reads μDrop™ and μDrop Duo Plates for micro-volume DNA/RNA and protein analysis
• Separate optimized measurement modes for absorption and turbidimetric measurements
• Allows kinetic, spectral, and endpoint measurements for a variety of applications
• Fast operation: full spectrum of a well is done in less than 10 seconds; typical DNA spectrum can be measured in less than three seconds and a full 96-well microplate is read with one wavelength in 6 seconds
• Performs onboard shaking and incubation for temperature-critical or cell-based assays
• Touchscreen models have an easy-to-use interface for stand-alone use, and includes ready-made protocols for UV based nucleic acid and protein quantification as well as colorimetric protein quantification
• Thermo Fisher Connect or Microsoft OneDrive cloud-based tools allow you to securely store, access, share, and manage your photometric data remotely (touchscreen models)
• Multiple languages available; choose between English, German, French, Spanish, Italian, Portuguese, Russian, Chinese, and Japanese (touchscreen models)
• Setting up of more demanding assays is easy via the powerful and intuitive SkanIt™ Software
• Multi-instrument software platform compatible with all Thermo Scientific microplate readers
• Open license software for unlimited installation to multiple computers
• Research Edition for life sciences; Drug Discovery Edition with features for compliance with FDA 21 CFR Part 11
• User-interface languages available: English, French, German, Spanish, Portuguese, Italian, Russian, Japanese, and Chinese
• Searchable SkanIt Cloud Library with ready-to-use protocols including calculations and true application example data
• Measurement sessions are easy to setup and navigate
• Intuitive Virtual Pipetting Tool assists in defining the plate layout

Multiskan SkyHigh Microplate Spectrophotometer

Easy-to-use monochromator-based spectrophotometer

Discover the Multiskan SkyHigh Microplate Spectrophotometer, a versatile UV/Vis microplate spectrophotometer engineered for ease of use across a broad spectrum of photometric research applications. Whether you’re analyzing DNA, RNA, proteins, or measuring turbidity, this microplate reader is designed to meet your needs.

The Multiskan SkyHigh system is available in various configurations, offering the flexibility required to adapt to diverse laboratory demands. Choose from touchscreen models that function as stand-alone instruments or for those who prefer PC-based operations, a dedicated Multiskan SkyHigh model operated exclusively via SkanIt Software is also available. Additionally, select models include cuvette reading capabilities when higher levels of precision are required or sample availability is limited.

With an array of options and applications, the Multiskan SkyHigh Microplate Spectrophotometer helps ensure you maximize the lab’s potential.

Features

Rapid measurement of low sample volumes

Thermo Scientific µDrop and µDrop Duo Plates are designed for rapid measurement of low sample volumes. You can analyze up to 32 samples simultaneously and quickly and easily wipe off the samples for serial measurements.

Thermo Fisher Connect and Microsoft OneDrive cloud-based capabilities enable users to access, manage, and share data remotely, available on touchscreen models.

SkanIt software can be used to control the Multiskan SkyHigh spectrophotometer and supports optimal use of the instrument’s features with a visual workflow, easy data analysis, and exporting capabilities.

Flexibility

Fast operation

Full spectrum (200–1,000 nm) of a well is obtained in less than 10 seconds and a full 96-well microplate is read in 6 seconds.

Frequently asked questions

Can you please let me know which plates with and without lid are compatible with the Multiskan SkyHigh Microplate Spectrophotometer?

The Multiskan SkyHigh Microplate Spectrophotometer supports clear bottom 6- to 48-, 96- and 384-well microplates with and without lids, where the maximum height of the microplate or a combination of the microplate and lid is 19.5 mm. Please refer to the Plate Selection Table for Plate Readers brochure (https://assets.thermofisher.com/TFS-Assets/BID/Flyers/nunc-plate-selection-table-plate-readers-flyer.pdf) for specific plates to use.
You can also see the specific plate types in SkanIt Software v7.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which is the best DNA or RNA quantification instrument?

All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

What is the difference between UV and fluorescence DNA quantification? Which DNA quantification technology should I choose?

UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn’t as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.